Biotechnology and Biological Sciences Doctoral Training Programme


Modifying membranes with mycolic acids – models, and experiments to understanding properties of unusual lipid functionality

Lab rotation project description

This project will offer three lab rotations; one per partner.

Rotations will be offered by each partner:

Engineering, Croft/Jäger

This rotation project will develop skills in working with our crowd-sourced world community grid computing environment (in conjunction with IBM) to probe the conformations of a defined set of mycolic acids, in preparation for developing the membrane models.

Training in molecular dynamic simulation, simulation analysis, and bioinformatics relating to structural analysis will be provided as part of this rotation.

School of Life Sciences, Bonev/Chemistry, Hirst

Solid state NMR mini-project: The objective is to give in a nutshell a flavour of the continuity in biological research from design and sample preparation through analysis to functional characterisation.

  • Week 1-2: A set of mycolic acid structures will be built in silico and computational predictions of their 13C NMR spectra will be obtained.
  • Week 3-4: Model membranes will be prepared form lipid mixtures mimicking mycobacterial membranes and incorporating either synthetic or natural mycolic acids. The membranes will be studied by solid state NMR to obtain a 13C spectrum from the MA.
  • Week 5-6: Experimental spectra will be compared to the prediction and used for spectral resonance assignment.

School of Life Sciences, Kovacs

To develop live models, expression systems for mycolic acids will need to be developed. This project will take the early steps in developing appropriate expression systems, taking a systems view of MA production.

Training in synthetic and systems biology techniques will be provided.

Fact file

Research theme

Industrial Biotechnology and the Bioeconomy

Location

Faculty of Engineering, School of Life Sciences and Centre for Biomolecular Sciences

Rotation

LR1, LR2 and LR3

Contact

Anna Croft – Engineering
anna.croft@nottingham.ac.uk

2nd supervisor

BBSRC Doctoral Training Partnerships

Linked PhD Project Outline

Mycolic acids are extremely long-chain (60-90 carbons) fatty acids that are produced by Mycobacteria, Corynebacteria and Norcardia.

They have been identified as strong contributors to the robustness of these organisms, including acting as a barrier to the transport of certain metabolites (including drug molecules) and protecting against external stressors such as damaging reactive oxygen species.

Biotechnology and Biological Sciences Doctoral Training Programme

The University of Nottingham
University Park
Nottingham, NG7 2RD

Tel: +44 (0) 115 8466946
Email: bbdtp@nottingham.ac.uk

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